Contributors:Rodolfo Romanach, Román-Ospino Andrés D., Scicolone James V., Alvarado-Hernandez Barbara B., Ortega-Zuniga Carlos
This data set includes the data for calibration, testing and validation of a model developed at a research laboratory and used to establish blending specifications at a development continuous manufacturing plant. This information would be later on implemented in a continuous manufacturing plant.
Contributors:Lu Bin, Liu Yan-jie, Wan Yingchun, Zheng Dan, Ma Hui-ting et al
Neomycin, an aminoglycoside antibiotic widely used in animal breeding industry, is difficult to detect due to its lack of significant UV absorbance or fluorophore. In this paper, a selective fluorescent sensor was developed to detect neomycin in complex biological samples. Fluorescent molecularly imprinted polymers (fMIPs) containing both imprinted cavity and boronate affinity site were synthesized on the surface of silica modified quantum dots (QDs). The achieved fMIPs exhibited high selectivity to neomycin by having two binding sites for neomycin. The existence of neomycin analogues (competing for the imprinted cavity) and D-glucose (competing for the boronate affinity site) did not affect the selective binding of neomycin to the fMIPs. A selective fluorescent sensor was developed by combining the fMIPs with a fluorescent microplate reader. The sensor could quantify neomycin in a linear range of 2-1000 μg/L with a detection limit of detection as 0.16 μg/L. Trace neomycin in complex biological samples after simple (one step) sample pretreatment, which were not suitable for HPLC-FLD analysis due to the presence of large amounts of interferences, could be quantified accurately by the fMIP sensor. The developed fMIP sensor provides a selective, sensitive, accurate and high through-put solution to detect neomycin in complex biological samples.
As far as we known, this is the first report to utilize QDs-based fMIPs with doubly selective binding sites to realize rapid and high through-put neomycin monitoring in complex biological samples.