The data files here included are simulated data, the ranges for which were based on data from studies of C57BL6J mice, previously analyzed and published. The files include one data set of N = 180 from 30 litters, 3 treatment groups, 10 litters per treatment, 6 mice per litter, with equal numbers of males (n = 3) and females (n = 3) per litter. Also provided are 4 data sets of N = 60 each, that were drawn from the larger data set of N = 180, and which include 1 "representative" male and 1 "representative" female per litter. Each data set includes five variables: mouse ID, litter, sex, group, and body weight at post-natal day 21. *These datasets were created solely for the purpose of demonstrating differences in statistical modeling when litter clusters complicate data analysis. Please see complete manuscript for a full discussion of the topic.

These excel files contain the raw data for each animal and each endpoint. Each excel file contains 1 worksheet for chemical information and another 6 worksheets, named as “Run A”, “Run B”, “Run C”, “Run D”, “Run E”, “Run F”, which correspond to different plate orientations (See the PDF). Eighteen chemicals were screened twice in two independent screens, one as a subset of the NTP 87-compound library, which we previously reported on (screen 1; (Zhang et al., 2018)), and the other as a flame retardant library (screen 2). Each screen contained 3 replicate runs with 3 different orientations of concentrations. The 8 animals in one row were exposed to the same concentration. In screen 1, chemical #1 – 8 were screened in 3 replicates of run A, B and C; chemical #9-18 were screened in 3 replicates of run B, D and E (for details, see (Zhang et al., 2018)). In screen 2, chemical #1 – 8 were screened in 3 replicates of run D, E and F; chemical #9-18 were screened in 3 replicates of run A, C and F. All worksheets contain the raw data in adult and regenerating (tail) planarians, except that the worksheets in “Eye regeneration - day 7 - FR. xlsx” only contain regenerating planarian data as eyes were not evaluated in full worms. In both sections (regenerating tails and adult worms), each column refers to one chemical, and each row represents one well in the 48-well plate with tested chemical concentrations labeled.