Microbiological Research

Data from manuscript "Biotic and abiotic factors affect Microcystin-LR concentrations in water/sediment interface" to ensure the transparency of publication.

The primers involved in this manuscript Genotyping and population diversity of Bacillus anthracis in China based on MLVA and canSNP analysis, UPGMA dendrogram of MLVA27 analysis and strains information from GenBank database were provided in the Supplemental files. Geographic information of all isolates and copy number of 27 VNTR loci were provided in the Supplemental files Table S8.

all of datas

The dataset contains supplementary data files for the manuscript titled "Legacy of a Pleistocene bacterial community: Patterns in community dynamics through changing ecosystems."

This study reports the introduction of egfp or mCherry markers to the Sp245, Sp7, and M40 wild-type strains of Azospirillum brasilense and the hhkB (encoding for a putative hybrid histidine kinase) minus mutant an isogenic strain of A. brasilense Sp245 to monitor colonization of wheat (Triticum aestivum). Two plasmids were constructed: (1) the pJMS-2 suicide plasmid derived from pSUP202 and harboring the mCherry gene expressed under the constitutive kanamycin resistance promoter to create a cis tag and (2) the broad-range plasmid pMP2449-5 that carries the mCherry gene under the lac promoter, which is derived from the plasmid pMP2444; to create the in trans tag. The stability of the plasmids encoding egfp and mCherry were confirmed in vitro for seven days of bacterial growth, and then, the A. brasilense strains harboring the plasmids were studied under nonselective conditions for adherence to seeds and, at seven or 14 days post-inoculation, for wheat root colonization. The utility of the labeled strains was proven by observation, using fluorescence microscopy and confocal laser scanning microscopy (CLSM) in wheat plants inoculated with the labeled strains and compared with the CFU g–1 for seed and wheat root. The method was suitable for observation of the in situ formation of mini-colonies, enabled visualization of bacterial colonization sites on large root fragments, and showed adherence to germinated seeds and root colonization of all strains by cell counts and direct microscopic examination. Thus, we are able to quantify the structures of the biofilms formed by each strain.

Table S1: Coordinates and Group Descriptions for the Genomic Neighborhoods of ATPS-containing GIs in Marine Cyanobacteria Table S2: Coordinates and Group Descriptions for the Genomic Neighborhoods of FBA I-containing GIs in Marine Cyanobacteria Table S3: List of Open Reading Frames in FBA I-containing GI in Prochlorococcus strain scB243_495I8 The position of each ORF is given, along with its reading frame. Each ORF has the top cyanophages that it shares similarity with indicated, based on the top BLAST hits, listed in decreasing order. In parentheses is the % similarity shared with a homolog from that cyanophage. Possible functions are also prescribed, based on the top BLAST hit for which a function has been given. E-Values for these hits are indicated.