Developmental Cell

Raw and processed data for all figures in Cell cycle heterogeneity can generate robust cell type proportioning (Dev. Cell, Gruenheit et al. 2018). Each Excel spreadsheet contains the data for one figure, different tabs contain data for subfigures. Three additional figures were also added. The word file contains the figure legends also for these additional figures.

Low temperatures affect plant growth, development, productivity, and ecological distribution. Expression of the C-repeat-binding factor (CBF) transcription factors is induced by cold stress, which in turn activates downstream cold-responsive (COR) genes that are required for the acquisition of freezing tolerance. Inducer of CBF expression 1 (ICE1) is a master regulator of CBFs, and ICE1 stability is crucial for its function. However, the regulation of ICE1 is not well understood. Here, we report that mitogen-activated protein kinase 3 (MPK3) and MPK6 interact with and phosphorylate ICE1, which reduces its stability and transcriptional activity. Consistently, the mpk3 and mpk6 single mutants and the mpk3 mpk6 double mutant show enhanced freezing tolerance, whereas MPK3/MPK6 activation attenuates freezing tolerance. Phosphor-inactive mutations of ICE1 complement freezing sensitivity in the ice1-2 mutant. These combined results indicate that MPK3/MPK6 phosphorylate and destabilize ICE1, which negatively regulates CBF expression and freezing tolerance in plants.

This example dataset is associated with the article "Quantitative multiscale cell imaging in controlled 3D microenvironments" by the same authors published in Developmental Cell in February 2016. These data are used by three Matlab functions (exampleCollagen3D.m, exampleBleb3D.m, exampleSurfaceIntensity3D.m) in the publication's "meSPIM Supplemental Information" zip file to present the computational workflow described in the article. Please see the publication for more information about this dataset.