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  • geoderma-Enzymatic stoichiometry reveals phosphorus limitation-induced changes in the soil bacterial communities and element cycling: Evidence from a long-term field experiment
    Its the original data of this article
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  • Data for: Molecular identification and characterization of pig's Cdh16 gene
    IHC staining of cadherin16 in a 3-month-old piglet's kidney.
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  • Chiral fungicide penconazole: absolute configuration, stereoselective bioactivity, toxicity, and environmental behavior in apple
    Chiral pesticides are existing two mirror images called enantiomers. In spite of their similar elements and structures, enantiomers of chiral pesticides were reported that could have different environmental behavior, bioactivities, toxicities, and metabolic characteristics when exposure of them to nature. Penconazole is a systemic fungicide of the triazole family. Up to now, the absolute configuration is undetermined. The bioactivity and toxicity of penconazole at stereomeric level have not been reported. In the present study, the analysis method of penconazole enantiomers by UPLC-MS/MS was optimized and a rapid quantitative analytical method was established. The work down systematically explored the absolute configuration, bioactivity, ecotoxicity, and environmental behavior of chiral fungicide penconazole. The absolute configuration of penconazole was confirmed by electronic circular dichroism (ECD). The acute toxicity toward Daphnia magna (Daphnia magnastraus). The enantioselective biological activities of penconazole enantiomers and raceme against four target pathogenic fungi, Alternaria alternate, Botryosphaeria dothidea, Colletotrichum gloeosporioides and Fusarium oxysporum. Stereoselective degradation test of penconazole in apple was conducted with bagging and non-bagging treatment among three regions in China. This results are expected to promote penconazole enantiomers application to improve pesticide effectiveness and to alleviate environmental pollution. And More accurate risk assessment will be analyzed to guarantee food security. The two stereoisomers of penconazole were separated by UPLC-MS/MS system with a Lux Cellulose-2 column (150 mm ×4.6 mm, 5 μm). 0.1% formic acid in methanol and 10 mmol/L ammonium acetate in water as the mobile phase. The effectiveness of the mobile phase ratios, column temperatures and flow rates were evaluated. The mobile phase A/B: 80/20, column temperature: 30 °C, flow rate: 0.25 ml/min were eventually chosen. The retention time of penconazole stereoisomers was about 8 min, less than half of previous detection time, and the RS was 1.75 indicating completely separation of penconazole stereoisomers. The absolute configuration of penconazole stereoisomers was confirmed by comparing experimental circular dichroism (CD) spectra with theoretical electronic circular dichroism (ECD) spectra. The three apple-producing areas in China (Liaoning province, Shandong province, Shanxi province) were picked on as open filed location. The fungicidal activity of penconazole and its stereoisomers were evaluated toward four target pathogens: Alternaria alternate, Botryosphaeria dothidea, Colletotrichum gloeosporioides and Fusarium oxysporum, which were cultivated in PDA medium. The acute toxicity tests of rac-penconazole and its two stereoisomers were carried out with Daphnia magna under the Test guidelines on environmental safety assessment for chemical pesticides: Daphnia magna acute immobilization test.
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  • Microbial phosphorus limitation under long-term fertilization induced changes in community structure and element cycle
    The data include soil physical and chemical properties, soil enzyme activity and stoichiometry, PLFA component abundance, soil bacterial diversity and abundance, and functional gene abundance predicted by picrust2. Including each data point, mean value and standard deviation.
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  • Data for: Risk Reduction of Chlorfenapyr Residue in tea planting, tea processing, and tea brewing
    Data and charts used in this article. Figure 1 The process of making green tea and black tea Figure 2 Degradation dynamics of chlorfenapyr in tea shoots at three locations Figure 3 Residue levels of chlorfenapyr in tea processing. Figure 4 Leaching rate of chlorfenapyr in the three infusions.
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  • Data for: Enhanced water extraction with high-pressure carbon dioxide on purple sweet potato pigments: comparison to traditional aqueous and ethanolic extraction
    This excel set includes the raw data of pigments yield, monomeric anthocyanins (half life calculation included), polymeric color, color, and the calculation of %peak area of pigments HPLC profile.
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  • Data for: Pkweb: an online application for toxicokinetic data analysis
    The data use for validating our model
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  • Data for: Identification and fruit expression analysis of peach abscisic acid signal transduction genes
    Protein sequences of genes involved in ABA signal transduction from tomato, Arabidopsis and Peach.
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  • 16S sequenceing date for cecal microbiota of pullets under acute heat stress
    Heat stress in poultry is deleterious to productive performance. Many studies have reported that chlorogenic acid (CGA) has positive effects in attenuating heat stress. This study examined the effects of dietary supplementation with CGA on the intestinal health and cecal microbiota composition of young hens challenged with acute heat stress. 100-day-old Hy-line brown pullets were randomly divided into four groups. The control group (C) and heat stress group (T1) received a basal diet. T2 group and T3 group received a basal diet supplemented with 300 mg/kg and 600 mg/kg CGA respectively, for two weeks before acute heat stress exposure. Pullets of T1, T2, and T3 group were exposed to 38℃ for 4 h while the control group was maintained at 25℃. 16 S rDNA Gene Sequencing for Cecal Microbiome was performed an Illumina Miseq.
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  • Data for: Correlation analysis of rhizosphere microbial diversity and soil physical-chemical properties provides insight into fertilization management during potato cultivation
    16S and ITS Sequencing
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