pMAD quantification

Published: 20 October 2022| Version 1 | DOI: 10.17632/278c6zx8c6.1
Contributor:
Peter Whitney

Description

Contains .csv files that were generated from analyzing pMAD microscopy images using Imaris. Also contains R script for generating graphs for the width of pMAD domain and intensity profiles of each genotype.

Files

Steps to reproduce

Data was collected by staining NC14 Drosophila embryos with anti-pMAD antibodies. Images were processed using Imaris by collected the nuclear position and intensity of pMAD staining. R script is used to generate plots from that data. Make sure file paths are correct before running, and appropriate packages are installed (check the top of the R script for which libraries are used.)

Institutions

New York University

Categories

Microscopy, Drosophila

Licence