AutoSpectral: 40-colour ID7000 dataset
Published: 27 October 2025| Version 1 | DOI: 10.17632/2kvc98hc65.1
Contributors:
Oliver Burton, , , , , Description
Healthy human PBMC and buffy coat samples, cryopreserved and stained with a 40-colour flow cytometry panel. Acquired on a 5-laser Sony ID7000 spectral instrument. Unmixed using WLSM on the Sony software or using AutoSpectral.
Files
Steps to reproduce
Contact Stephanie Humblet-Baron for the staining protocol. For unmixing, see instructions on the GitHub repository for AutoSpectral.
Institutions
University of Cambridge, Katholieke Universiteit Leuven
Categories
Flow Cytometry, Spectral Unmixing