AutoSpectral: 40-colour ID7000 dataset
Published: 27 October 2025| Version 1 | DOI: 10.17632/2kvc98hc65.1
Contributors:
Oliver Burton, , , , , Description
Healthy human PBMC and buffy coat samples, cryopreserved and stained with a 40-colour flow cytometry panel. Acquired on a 5-laser Sony ID7000 spectral instrument. Unmixed using WLSM on the Sony software or using AutoSpectral.
Files
Steps to reproduce
Contact Stephanie Humblet-Baron for the staining protocol. For unmixing, see instructions on the GitHub repository for AutoSpectral.
Institutions
University of Cambridge, Katholieke Universiteit Leuven
Categories
Flow Cytometry, Spectral Unmixing
Funding
European Research Council
No 874707 (EXIMIOUS)
KU Leuven
C1 Program
UK Research and Innovation
MR/Y004450/1 (IMMPROVE)
Wellcome Trust
222442/A/21/Z