Astrocyte-derived extracellular vesicles enhance the electrophysiological function and cell survival of human cortical neurons in vitro

Published: 23 Jan 2020 | Version 1 | DOI: 10.17632/2kw7tgph94.1
Contributor(s):

Description of this data

The immunostained image analysis of neurons created, NTA analysis of obtained EVs, image-based demonstration of EV-uptake by neurons, FACS analysis to quantify apoptotic behavior of cultures of interest, beta-galactosidase result to explain cell senescence status in each group, patch clamp experiment result to demonstrate the electrophusiological effect of astroglial EV treatment.

Experiment data files

  • Axon growth_BF images
    Cite
    • 1_10
      Cite
      • 12hrs_pp
        Cite
      • 2hrs_post_plating_exo_treated here
        Cite
      • 4hrs_pp
        Cite
    • 1_100
      Cite
      • 12hrs_pp
        Cite
      • 2hrs_post_plating_exo_treated here
        Cite
      • 4hrs_pp
        Cite
    • 1_25
      Cite
      • 12hrs_pp
        Cite
      • 2hrs_post_plating_exo_treated here
        Cite
      • 4hrs_pp
        Cite
    • 1_250
      Cite
      • 12hrs_pp
        Cite
      • 2hrs_post_plating_exo_treated here
        Cite
      • 4hrs_pp
        Cite
    • Confocal images
      Cite
    • Untreated
      Cite
      • 12hrs_PP
        Cite
      • 2hrs_post_plating_exo_treated here
        Cite
      • 6hrs_pp
        Cite
  • Flow cytometry
    Cite
    • 100219-Exosome
      Cite
  • Immunostaining images
    Cite
    • 20190213
      Cite
    • 20190215
      Cite
    • 20190307
      Cite
      • Astrocyte and Schwann cells_heterogeneity test
        Cite
        • hAstrocyte
          Cite
        • hSchwann cell
          Cite
      • Astrocytes on 5%_FL_Nafion
        Cite
      • Astrocytes on 5%_NafionP
        Cite
    • 20190408
      Cite
      • NGN2_Neurons (d4)_5%Nafion alignment_16 dfferent patterns
        Cite
        • 16
          Cite
        • 5
          Cite
      • NGN2_Neurons (d4)_CUX1 expression
        Cite
    • 20190505
      Cite
      • 20190506_Exoglow_test_1
        Cite
    • 20190702
      Cite
    • 20190703_CUX1 expression_60x
      Cite
    • 20190703_Exosome_uptake_60x
      Cite
      • 1_100 (exosome to cell ratio)
        Cite
      • 1_25 (exosome to cell ratio)
        Cite
      • 1_250 (exosome to cell ratio)
        Cite
    • 20190717_hAC exosome_intensity comparison
      Cite
    • 20190928_low density axon length
      Cite
  • Nanosight_NTA assay
    Cite
    • 12hrs_incubation
      Cite
    • 24hrs_incubation
      Cite
    • 36hrs_incubation
      Cite
    • 48hrs_incubation
      Cite
    • 84hrs_incubation
      Cite
    • hAC_made by Changho_on_190208
      Cite
    • Incubation time vs. Exosome density
      Cite
      • 12hrs_incubation
        Cite
      • 24hrs_incubation
        Cite
      • 36hrs_incubation
        Cite
      • 48hrs_incubation
        Cite
      • 84hrs_incubationi
        Cite
  • Patch clamp assay
    Cite
    • 20190305_0306-NGN2 test1 D28
      Cite
      • 20190305 - NGN2 test 1 D28 (raw data)
        Cite
      • AP trace
        Cite
      • APD_Up and Down_Slope
        Cite
      • Raw data
        Cite
    • 20190606 - NGN2 test 1 D14
      Cite
    • 20190620_0621-NGN2 test2 D28
      Cite
    • 20190912_NGN2 test 3 D28_consolidated
      Cite
      • Raw data
        Cite
        • 20190620
          Cite
        • 20190912
          Cite
  • Senescence assay
    Cite
    • 20190711-beta-gal test_@day4 (24 hrs after 3days diff)
      Cite
    • 20190717-beta-gal test_various timepoints with exosome
      Cite

Latest version

  • Version 1

    2020-01-23

    Published: 2020-01-23

    DOI: 10.17632/2kw7tgph94.1

    Cite this dataset

    Chun, Changho; Smith, Alec; Kim, Deok-Ho (2020), “Astrocyte-derived extracellular vesicles enhance the electrophysiological function and cell survival of human cortical neurons in vitro ”, Mendeley Data, v1 http://dx.doi.org/10.17632/2kw7tgph94.1

Statistics

Views: 1608
Downloads: 302

Institutions

University of Washington

Categories

Confocal Microscopy, Flow Cytometry, Patch Clamp Technique

Licence

CC BY 4.0 Learn more

The files associated with this dataset are licensed under a Creative Commons Attribution 4.0 International licence.

What does this mean?
You can share, copy and modify this dataset so long as you give appropriate credit, provide a link to the CC BY license, and indicate if changes were made, but you may not do so in a way that suggests the rights holder has endorsed you or your use of the dataset. Note that further permission may be required for any content within the dataset that is identified as belonging to a third party.

Report