Dataset on fungal communities in common reed
Description
Dataset contains results of shotgun metagenomes of fungal colonisers of common reed leaves and litter in different habitats.
Files
Steps to reproduce
In the current dataset, the whole community DNA was extracted from common reed leaves using the GenElute® Plant Genomic DNA isolation kit (Sigma) following step-by-step procedures from the manufacturer's manual. Shotgun metagenomic sequencing was done via the Illumina HiSeqX platform (2 x 150 pair-ends) according to the manufacturer's guidelines. Analysis and annotation of output data were performed through Metagenomics rapid annotation (MG-RAST) online server (Meyer et al., 2008) with the default parameters. Following quality control (QC), sequences were annotated using BLAT (a BLAST-like alignment tool) algorithm (Kent, 2002) against M5NR database (Wilke et al., 2012), which offers non-redundant integration of numerous databases.