Persistent Trajectory Modulated Hippocampal Neurons Dataset

Published: 14 May 2020| Version 1 | DOI: 10.17632/2twf9f834v.1
Contributor:
Nat Kinsky

Description

This repository contains processed imaging and behavioral data used in "Trajectory-modulated hippocampal neurons persist throughout memory-guided navigation" by Kinsky, N.K, Mau, W., Sullivan, D.W., Levy, S.J., Ruesch, E.A., and Hasselmo, M.E.. Nature Communications (2020). Due to its size, raw data is housed on a different repository and will be made available upon reasonable request, e-mail Nat Kinsky (nat[dot]kinsky[at]gmail[dot]com). See also the Recording and Upload Summary.xlsx file for immediate access to a subset of raw data. Folders are provided for each imaging session. In the case that two imaging sessions were performed, the first session's folder will have a '- 1" appended after the date and the 2nd will have a "- 2" appended. Recording and Upload Summary.xlsx - tracks all recording sessions with notes about each. See notes for session specific information, and contact nat[dot]kinsky[at]gmail[dot]com with any questions. MakeMouseSessionList.mat - contains additional information about each recording including the pixel_to_cm value necessary to convert the position data in xpos_interp and ypos_interp to centimeters. All variables are saved in the processed_minimum.mat MATLAB file. Animal, Date, Session: Mouse name, date of session, and session number on a given day. Verify these match the data folder before proceeding! PSAbool - n neurons x n timestamps (Sample Rate = 20 fps) boolean of putative spiking epochs (rising phase of calcium trace above a threshold attributed to that neuron by Tenaspis v4, https://github.com/SharpWave/TENASPIS). Since the sample rate is 20fps for each movie, the first column corresponds to neuron activity at 0.05s, 2nd at 0.10s, etc. raw_trace - n neurons x n timestamps (Sample Rate = 20 fps) of fluorescence within each neuron ROI. NeuronImage - n neurons cell with boolean the size of the imaging window identifying which pixels are attributed to each neuron min_proj - single array the size of the imaging window with the minimum fluroescence values recorded in that imaging session. Used for between session alignment of neurons/imaging window. time_interp, xpos_interp, ypos_interp: arrays tracking behavioral time and x/y position in PIXELS interpolated to imaging timestamps. time_interp = 0.05 corresponds to the first value in PSAbool. Track dimension are 64cm (x direction) by 29cm (y direction) with each corridor ~7.5 cm wide. t_start_free: in order to break Mouse 4 out of a perseveration habit he developed halfway through the experiment he was forced to alternate at the beginning of a subset of sessions. This variable notes the time from the "time_interp" variable when the animal stopped being forced to alternate and was allowed to freely alternate.

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