Multifunctional material enables ultrafast and large-scale enrichment of glycopeptides and reveals distinct spatial patterns in oxidative stress

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Our study offers a convenient and robust site-specific chemproteomics tool for glycoproteome profiling, enabling simultaneous identification of O-GlcNAc sites and N-glycosites by a two-step enzymatic release strategy, and the application of this material revealed distinct spatial O-GlcNAcylation patterns upon oxidative stress.

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