NCOMMS-23-00463

Description

Sanger sequencing reads from genomic DNA (isolated from whole blood) from individuals with inflammation and autoimmunity.

Steps to reproduce

Genomic DNA was isolated (Qiagen Cat No. 51304) from ficoll-isolated granulocytes from whole blood of each subject, then amplified by PCR using targeted JAK1 probes. PCR products were then purified (ThermoFisher Scientific Cat No. K0831) and Sanger sequenced using targeted primers and analyzed on the using Snapgene (v4.2.11).

Institutions

• Icahn School of Medicine at Mount Sinai

  NY, New York

Categories

Funders

• National Institute of Allergy and Infectious Diseases

  Maryland, United States

  Grant ID: 5R01AI148963

Licence