NCOMMS-23-00463
Published: 23 August 2023| Version 1 | DOI: 10.17632/2vfgyfyjfh.1
Contributors:
Michael Horesh, Description
Sanger sequencing reads from genomic DNA (isolated from whole blood) from individuals with inflammation and autoimmunity.
Files
Steps to reproduce
Genomic DNA was isolated (Qiagen Cat No. 51304) from ficoll-isolated granulocytes from whole blood of each subject, then amplified by PCR using targeted JAK1 probes. PCR products were then purified (ThermoFisher Scientific Cat No. K0831) and Sanger sequenced using targeted primers and analyzed on the using Snapgene (v4.2.11).
Institutions
Icahn School of Medicine at Mount Sinai
Categories
Sanger Sequencing
Funding
National Institute of Allergy and Infectious Diseases
5R01AI148963