Multiple sequence alignment of the family Polystomatidae, based on the markers 12S, 18S, 28S and cox1. This is the alignment used to construct the phylogeny of Polystomatidae in the paper "Amended diagnosis, mitochondrial genome, and phylogenetic position of Sphyranura euryceae (Neodermata, Monogenea, Polystomatidae), a parasite of the Oklahoma salamander."
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The four sequence sets (12S, 18S, 28S and cox1) were aligned per marker using MAFFT v.7.310 and trimmed using TrimAl v.1.2 “strict mode''. Selection of trimming criteria was based upon visual inspection in AliView v.1.28. The four separate alignments were then concatenated into a single alignment using the script concat.py v.0.21 (https://github.com/reslp/concat).