Target predictions for abundantly secreted nematode microRNAs
Host (mouse) targets of the 20 most abundantly secrected miRNAs of Heligmosomoides polygyrus bakeri, Trichuris muris, and Ascaris suum were predicted computationally with TargetScan v.7.
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Secreted miRNA lists from H. polygyrus bakeri, T. muris, and A. suum were retrieved from the literature (Hansen et al. 2019 (doi: 10.1080/20013078.2019.1578116); Eichenberger, Talukder, et al. 2018 (doi: 10.1080/20013078.2018.1428004); Tritten et al. 2017 (doi: 10.1016/j.exppara.2017.05.003); Buck et al. 2014 (doi: 10.1038/ncomms6488)). Target prediction in the mouse genome for the top 20 most abundant miRNAs was performed as described previously (Duguet et al. 2020) using the TargetScan software package v. 7 (http://www.targetscan.org), using default parameters (Agarwal et al. 2015 (doi: 10.7554/eLife.05005)).