Experimental design and response surface analysis data for optimization of ultrasound-assisted acid hydrolysis and HPLC-UV determination of amino acids in plant protein isolates
Description
This dataset contains source experimental and statistical data supporting the study “Rapid and efficient high-performance liquid chromatography-ultraviolet determination of total amino acids in protein isolates by ultrasound-assisted acid hydrolysis” by Custodio-Mendoza et al. (2024), published in Ultrasonics Sonochemistry, Volume 111, Article 107082 (DOI: 10.1016/j.ultsonch.2024.107082). The study developed, optimized, and validated a rapid ultrasound-assisted acid hydrolysis procedure coupled with high-performance liquid chromatography with ultraviolet detection (HPLC-UV) for the determination of total amino acids in plant-based protein isolates. The approach was designed as a faster and more resource-efficient alternative to conventional acid hydrolysis, which typically requires prolonged heating prior to chromatographic analysis. The deposited data originate from the experimental design and statistical optimization stages of method development. They contain graphical outputs used to evaluate the influence and relative importance of the investigated hydrolysis parameters on the analytical responses of individual amino acids. The files include standardized effect plots and related statistical outputs for selected amino acids, including asparagine (Asn), aspartic acid (Asp), glycine (Gly), lysine (Lys), serine (Ser), and valine (Val). The “Efectos totales” files present the estimated total effects of experimental factors and their interactions on the analytical response for individual amino acids. The “Pesos Delta” files provide complementary graphical outputs related to the statistical weighting and relative contribution of the investigated factors. Together, these files document the statistical analysis underlying selection of the experimental conditions used in the final analytical procedure. The optimization evaluated key variables affecting ultrasound-assisted protein hydrolysis. Ultrasound treatment was used to accelerate acid hydrolysis through acoustic cavitation and substantially reduce the required processing time. The optimized procedure involved hydrolysis for 30 min at 90 °C using 6 M hydrochloric acid. Following hydrolysis, amino acids were derivatized with 9-fluorenylmethyloxycarbonyl chloride (Fmoc-Cl) prior to HPLC-UV determination. The derivatization procedure was also optimized, and the complete analytical method was subsequently validated and applied to the determination of amino acid profiles in plant-based protein samples. The developed method demonstrated good analytical performance and showed no statistically significant differences compared with conventional acid hydrolysis, supporting ultrasound-assisted hydrolysis as a rapid alternative for total amino acid determination in plant protein materials.
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Institutions
- Warsaw University of Life SciencesMazovia, Warsaw