Immersion-administered lidocaine as an adjuvant to clove essential oil in Nile tilapia: raw data
Description
This dataset contains raw data from an immersion anesthesia study in juvenile Nile tilapia (Oreochromis niloticus) assessing clove essential oil (100 µL/L) alone or combined with lidocaine (60 or 80 mg/L). Data includes individual fish metadata (body weight, length, Fulton’s condition factor), anesthesia endpoints (time to vertical loss, time to anesthetic plane, and recovery time), and opercular respiratory rate recorded every 20 s for 180 s during induction and recovery.
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Steps to reproduce
Raw data were obtained from an immersion anesthesia experiment in juvenile Nile tilapia (Oreochromis niloticus). Fish were acclimated for one month in a greenhouse-based recirculating aquaculture system (RAS; six 300-L tanks sharing mechanical/biological filtration) under natural photoperiod. Water quality during acclimation was maintained at 27–28 °C, dissolved oxygen (DO) ≥ 5 mg/L, and pH ~ 8.2. DO was verified with a YSI Model 20 Plus meter and pH was measured with an EcoSense pH10 meter; ammonia was continuously monitored at the biofilter using a Seachem monitor. Fish were fed a commercial diet (45% crude protein) and fasted 12 h before each procedure. Fish with body weight < 80 g and/or Fulton’s condition factor K < 1.5 were excluded. Fish were randomly assigned to three immersion treatments (n=12 per treatment) and tested over three consecutive mornings, with all treatments represented each day to reduce day-of-sampling bias. All treatments used clove essential oil (CEO) at 100 µL/L as the base anesthetic. CEO was freshly diluted 1:10 (v/v) in 96% ethanol immediately before use (no stored stock). Lidocaine HCl (commercial solution, 20 mg/mL) was added to achieve: (1) CEO alone (control), (2) CEO + lidocaine 60 mg/L, and (3) CEO + lidocaine 80 mg/L. Each individual trial was conducted in a 20-L aquarium containing 10 L clean water at 27.5 ± 0.3 °C with aeration (BOYU D-300; nominal 1.2 L/min). Upon reaching the operational anesthetic plane, fish were removed for morphometrics and weighing, rinsed 5 s in clean water to reduce carryover, and transferred to a 10-L recovery aquarium with aeration (BOYU; max 3.2 L/min). Time endpoints were recorded with a stopwatch as seconds from immersion start using predefined criteria: (i) vertical loss (LOE; safety cut-off 2 min), (ii) time to anesthetic plane (no motor response to gentle tactile stimulation with blunt forceps at caudal peduncle/fin), and (iii) recovery time (regained and maintained vertical posture ≥20 s plus regular opercular breathing). Respiratory rate (RR) was quantified as opercular movements counted in consecutive 20-s windows and converted to movements/min (count×3), recorded serially over 180 s during induction/anesthesia and 180 s during recovery. Total length was measured with an ichthyometer and body weight with an Ohaus Scout SPX621 balance; Fulton’s K was calculated from length and weight. The Excel dataset includes: “Time” (fish ID, treatment, weight, length, K, and time endpoints; minutes and seconds), “Induction Freq” and “Recovery Freq” (raw RR per 20-s window in analysis-ready format), and “Graphics” (post-analysis summary outputs: LSmeans, SE, and 95% CI exported from Statgraphics 19 for figure preparation). Statistical analyses were performed in Statgraphics 19 using ANCOVA/GLM (treatment fixed, weight covariate) for time endpoints and repeated-measures GLM for RR trajectories (treatment, time, and treatment×time; fish nested within treatment).
Institutions
- Universidad de Guanajuato Division de Ciencias de la VidaGuanajuato, Irapuato