B cell analyses for PD-1- and PD-L1-deficient humans and PD-1 KO mice
Description
We tested the hypothesis that PD-1:PD-L1 signaling is essential for optimal B-cell memory and antibody responses by studying the recently described PD-1-deficient (Ogishi et al. Nat. Med. 2021) and PD-L1-deficient (Johnson, Ogishi, Domingo-Vila et al. JEM 2024) patients, as well as PD-1 KO mice. This repository contains processed data and analyses for (matched figures in the corresponding paper): - Single-cell trajectory analysis of B cells of the PD-1- and PD-L1-deficient patients (Fig. 2). - BCR repertoire analysis of sorted naive and memory B cells of the PD-1-deficient patient and whole-blood leukocytes of the PD-L1-deficient patients (Fig. S2). - Bulk RNASeq of in vitro stimulated PBMCs of the PD-1- and PD-L1-deficient patients (Fig. 4). - Bulk RNASeq of sorted naive and memory B cells of the PD-1-deficient patient and some disease controls (Fig. S2, 2, and 6). - Bulk RNASeq of sorted naive B cells from healthy donors stimulated in the presence of PD-1 or PD-L1-neutralizing antibodies (Fig. S5). - Bulk RNASeq of sorted naive and memory B cells from WT and PD1 KO mice (Fig. 3).
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Institutions
- Garvan Institute of Medical Research
- Kyoto Daigaku
- Rockefeller University