Porcine reproductive and respiratory syndrome virus upregulates SMPDL3B to promote viral replication by modulating lipid metabolism

Description

We generated a collection of porcine genome-scale CRISPR/Cas9 knockout (PigGeCKO) cells on a PAM background.

Steps to reproduce

Prior to screening, we isolated and identified nine strains of PRRSV, all of which were found to be HP-PRRSV. We examined PRRSV-induced cell death following infection at various multiplicity of infection (MOI) values (0.001, 0.01, 0.1, and 1) and identified the optimal titer for PRRSV-induced cell death in PAM cells (MOI = 0.01). We then performed three rounds of screening to identify predisposing factors that regulate PRRSV-induced host cell death.

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