Whole genome sequence data of Leptospira weilii and Leptospira Leptospira kirschneri isolated from human subjects Sri Lanka

Description

Leptospira kirschneri (3/38) and Leptospira weilii(4/23) in the NCBI database is limited. Sequencing the entire Leptospira genome and conducting thorough analysis of the data can significantly enhance the ability to predict and respond to outbreaks more quickly. Whole genome sequencing proves particularly valuable in the molecular epidemiology of infections, particularly during outbreaks, by improving efficiency and accuracy. • Researchers studying leptospirosis can benefit from this data by utilizing it in their studies related to vaccine development and uncovering the molecular mechanisms underlying pathogenesis in leptospirosis.

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Cultures were isolated using EMJH media DNA extraction was performed using the DNeasy Blood & Tissue Kit, following a Gram-negative bacteria protocol, with an RNase cleanup step included. The quantity of extracted DNA was measured using a Qubit 4 fluorometer. High-quality genomic DNA (gDNA) was utilized to construct multiplexed PacBio SMRTbell libraries using the SMRTbell Express Template Prep Kit. Shearing of 1 g of genomic DNA was achieved using Covaris g-tubes, and DNA concentration was enhanced using AMPure PB beads. The DNA underwent repair and ligation to a barcoded 8A adaptor, followed by adherence to size selection instructions for Blue Pippin TM 4 kb or more, Whole genome sequencing was conducted using the PacBio Single Molecule Real-Time (SMRT) platform Raw data were processing and genome assembly using Canu 2.1 and Circlator and then circularized. Genome annotation was conducted using RAST (Rapid Annotation Using Subsystem Technology ) and NCBI Prokaryotic Genome Annotation Pipeline.

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