The DNA damage checkpoint eliminates mouse oocytes with chromosome synapsis failure.Rinaldi et al

Published: 19 July 2017| Version 1 | DOI: 10.17632/3n2yfpk4vh.1
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Figure 1A) H&E stained histological sections of 8 weeks old ovaries, at lower and higher magnification. Spo11-/- (lable s--), Spo11-/-Chk2-/- (lable s--c--), and wildt type (lable wt) control Figure 2A) H&E stained histological sections of 8 weeks old ovaries, at lower and higher magnification. Trip13Gt/Gt (label T--), Trip13Gt/GtHormad2-/- (lable T--H2--), and Hormad2-/- (lable H2--) Figure 3A)H&E stained histological sections of 8 weeks old ovaries. Dmc1-/-Chk2-/- (lable d--c--), Dmc1-/-Hormad2-/- (lable d--h2--), and Dmc1-/-Chk2-/-Hormad2-/- (lable d--c--h2--) Figure 4A) Meiotic spreads of different mutants at different stages Figure 5A) Meiotic spreads of 4 hours and 8 hours after 2gys IR for the different mutants. Figure 6B) Immunofluorescence images of wild type ovaries submitted to: 0.1Gy (labled wt 0-1gy) and 0.2Gy (lable wt 0-2gy); and Chk2-/- submitted to 7Gy (labled Chk 7gy) or 8Gy (labled 8Gy).

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Photoshop allows for a nondestructive editing of images, thus images from spreads were uploaded as photoshop files (.psd). The uploaded files contain both the raw image as well as all the layers specifying each visual improvement used. We used photoshop's adjustment layers for contrast, brightness, etc in order to improve visibility.

Institutions

Cornell University

Categories

Histology, DNA Repair, Female Reproduction, Ovary, Meiosis, Food Spread, Oocyte Development

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