The original images of Western blotting , Imagine J batch processing macro code for Toluidine Blue and a database for Serum biochemical parameters of liver and kidney function in rats
Description
For the Western Blot experiments conducted in this study, the supplementary files contain the uncropped, raw protein band data. This includes the original bands from three biological replicates, which were utilized for statistical analysis. For specific code files of Toluidine Blue staining, please refer to the document "imagine J-Batch Processing Macro Code for Toluidine Blue". For blood biochemical parameters for hepatic and renal function in rats, please refer to the document"Serum biochemical parameters of liver and kidney function in rats".
Files
Steps to reproduce
Femur samples were pulverized into bone powder and subsequently lysed in RIPA buffer supplemented with protease and phosphatase inhibitors. After protein quantification, the samples were underwent separation on 7.5%-12.5% SDS-PAGE gels and were subsequently transferred onto PVDF membranes. The membranes were blocked with 5% skim milk in TBST for 2 hours at 37°C. Subsequently, the membranes were incubated overnight at 4°C with primary antibodies. On the following day, the membranes were incubated with secondary antibodies for 2 hours. Immunoreactive bands were identified utilizing Tanon 4600 ECL detection system (Shanghai, China). Protein expression levels subsequently analyzed using Image-J software (version 1.8.0).
Institutions
- Guangzhou Medical University