Raw data for "Multi-omics Profiling of Diabetes Mellitus Mouse Models"
Description
Raw data for the revised manuscript "Multi-omics Profiling of Diabetes Mellitus Mouse Models". The main content is the original data of identified metabolomics and proteomics..
Files
Steps to reproduce
LC‑MS/MS analysis was performed on a Thermo Fisher UHPLC‑Q Exactive HF‑X system (Shanghai Majorbio Bio‑Pharm Technology Co., Ltd.). The LC-MS/MS analysis of sample was conducted on a Thermo UHPLC-Q Exactive HF-X system equipped with an ACQUITY HSS T3 column (100 mm × 2.1 mm i.d., 1.8 μm; Waters, USA) at Majorbio Bio-Pharm Technology Co. Ltd. (Shanghai, China). The mobile phases consisted of 0.1% formic acid in water:acetonitrile (95:5, v/v) (solvent A) and 0.1% formic acid in acetonitrile:isopropanol:water (47.5:47.5, v/v) (solvent B). The flow rate was 0.40 mL/min and the column temperature was 40℃. The injection volume was 3 μL. A 3‑µL sample aliquot was separated on an HSS T3 column (100 mm × 2.1 mm i.d., 1.8 µm) prior to mass spectrometric detection. The mass spectrometric data were collected using a Thermo UHPLC-Q Exactive HF-X Mass Spectrometer equipped with an electrospray ionization (ESI) source operating in positive mode and negative mode. The optimal conditions were set as followed: source temperature at 425℃; sheath gas flow rate at 50 arb; Aux gas flow rate at 13 arb; ion-spray voltage floating (ISVF) at -3500V in negative mode and 3500V in positive mode, respectively; Normalized collision energy , 20-40-60V rolling for MS/MS. Full MS resolution was 60000, and MS/MS resolution was 7500. Data acquisition was performed with the Data Dependent Acquisition (DDA) mode. The detection was carried out over a mass range of 70-1050 m/z. Metabolites were first identified by matching MS/MS spectra against public databases (e.g., HMDB and KEGG), combined with accurate molecular weight and retention time information. Subsequent data processing—including preprocessing, metabolite annotation, differential metabolite screening, and pathway enrichment analysis—was performed using the integrated analytical modules of the in‑house metabolomics cloud platform developed by Majorbio Bio‑Pharm Technology Co., Ltd.