Comprehensive- analytical dataset on herbo-mineral formulation Chandramrita rasa: HPTLC fingerprinting with piperine estimation, FTIR Spectroscopy, SEM-EDS, XRD, CHNS-O, ICP-AES and AAS characterization
Description
This dataset provides a comprehensive pharmaceutico-analytical profile of Chandramrita Rasa (CHR), a classical Ayurvedic herbo-mineral formulation traditionally indicated for managing fever, cough, cold, bronchitis, asthma, and related respiratory disorders. To comprehensively characterize its phytochemical, elemental, physicochemical, morphological, and crystalline properties, CHR was evaluated using multiple complementary analytical techniques, including High-Performance Thin-Layer Chromatography (HPTLC), Fourier Transform Infrared Spectroscopy (FTIR), CHNS-O elemental analysis, Inductively Coupled Plasma–Atomic Emission Spectroscopy (ICP-AES), Atomic Absorption Spectroscopy (AAS), Field Emission Scanning Electron Microscopy coupled with Energy Dispersive X-ray Spectroscopy (FEG-SEM/EDS), and X-ray Diffraction (XRD). The HPTLC dataset includes chromatograms, densitograms, calibration data, peak profiles, and quantitative estimation of piperine, while FTIR provides characteristic spectral fingerprints representing major functional groups present in the formulation. CHNS-O, ICP-AES, and AAS datasets provide quantitative information on organic and inorganic elemental composition, including major and trace elements. FEG-SEM/EDS data provide morphological and elemental information, whereas XRD data facilitate evaluation of the crystalline characteristics and phase composition of the formulation. Advanced chromatographic, spectroscopic, spectrometric, microscopic, and diffraction-based approaches were integrated to generate a multidimensional analytical fingerprint of CHR. Key analytical parameters, including retention factors, peak characteristics, spectral absorption bands, elemental concentrations, morphological features, and diffraction patterns, are compiled to support interpretation and comparative evaluation. The dataset is structured to facilitate pharmaceutical characterization, quality control, authentication, analytical method development, and standardization of Ayurvedic herbo-mineral formulations. The compiled analytical data, including chromatograms, densitograms, spectra, elemental profiles, microscopic images, and diffraction patterns, provide a reproducible reference for researchers investigating the physicochemical and analytical characteristics of CHR. The dataset may further support comparative research, quality-assurance studies, computational analysis, and future investigations aimed at establishing standardized analytical profiles for classical Ayurvedic formulations.
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Steps to reproduce
Everything is explained in the Methodology section 1. Prepare Chandramrita Rasa according to the specified classical pharmaceutical procedure and document the raw materials, processing steps, and batch details. 2. Collect a representative sample of the final formulation and homogenize it appropriately before analysis. 3. For HPTLC analysis, prepare the methanolic extract of the sample, apply the extract to silica gel 60 F254 plates, and develop the plate using cyclohexane:ethyl acetate (5:3, v/v) as the mobile phase. Record chromatographic profiles at 254 and 366 nm and perform densitometric analysis. Use Piperine as the reference marker and determine its concentration using the validated calibration procedure. 4. For FTIR analysis, prepare the sample according to the selected FTIR sampling procedure and record spectra over the 4000–400 cm⁻¹ range. Identify and document the characteristic absorption bands. 5. For CHNS-O analysis, prepare the dried and homogenized sample according to the elemental analyzer manufacturer's requirements and determine the percentage composition of carbon, hydrogen, nitrogen, sulfur, and oxygen. 6. For ICP-AES and AAS analysis, digest accurately weighed samples using an appropriate validated acid-digestion procedure. Analyze the prepared solutions using calibrated instruments with suitable elemental standards, blanks, and quality-control samples. Record the concentrations of the target elements and the applicable detection limits. 7. For FEG-SEM/EDS analysis, prepare a representative powdered sample using the instrument-specific sample-mounting and coating procedure. Acquire high-resolution morphological images and corresponding EDS elemental spectra/maps. 8. For XRD analysis, prepare a finely powdered and homogenized sample, mount it uniformly on the sample holder, and acquire the diffraction pattern using the selected instrument conditions. Process the diffractogram to identify characteristic crystalline phases. 9. Perform appropriate quality-control checks, including blanks, reference standards, calibration verification, replicate measurements, and instrument-performance checks wherever applicable. 10. Compile the raw spectra, chromatograms, densitograms, peak tables, elemental results, microscopy images, EDS spectra/maps, and XRD patterns together with the corresponding analytical conditions and metadata.
Institutions
- All India Institute of Ayurveda, New DelhiDelhi, New Delhi