Numerical source data for OsSTP13-mediated blast resistance and yield-related traits in rice
Description
This dataset contains numerical source data for a study of the rice sugar transporter OsSTP13 in the Taipei 309 background. It includes Ct measurements for infection time-course expression, fungal biomass and selected-gene qPCR validation; lesion lengths; leaf-sheath infection-stage counts; agronomic observations; targeted metabolite concentrations; and author-reported RNA-seq relative expression values for selected genes. The original workbook is preserved alongside structured CSV files, an editable data workbook, a figure-to-file map, a data dictionary and an English README. Units, biological and technical replicates, calculations, coverage gaps and differences between the supplied records and current manuscript figures are documented. Raw sequencing reads and original image or instrument files are outside the scope of this workbook-derived dataset.
Files
Steps to reproduce
1. Read README.txt and Figure_Data_Map.csv to identify the files corresponding to each figure panel. Consult Data_Dictionary.csv for variables, units and source-cell references. 2. Inspect the unchanged original workbook and the structured CSV files. For qPCR calculations, subtract reference Ct from target Ct, subtract the relevant control mean delta Ct, and calculate relative expression as 2^(-delta_delta_Ct). 3. For fungal biomass, average the technical relative values within each biological replicate before calculating group summaries. 4. Calculate infection-stage percentages as stage count divided by total sites multiplied by 100. Convert seed-setting fractions to percentages by multiplying by 100. 5. Use the editable workbook or Group_summaries.csv to inspect sample counts, arithmetic means, sample standard deviations and standard errors. Reported RNA-seq values are retained from the supplied records and were not reconstructed from a count matrix. 6. Review the coverage notes and documented source-versus-figure differences before interpreting the data.