Source data for: Effect of seed length and binding motifs on Hfq-mediated sRNA-mRNA annealing analyzed using single-molecule FRET

Published: 24 March 2026| Version 3 | DOI: 10.17632/3z8fng2c8g.3
Contributors:
Maciej Dylewski, Ewelina Malecka

Description

This dataset contains raw and processed single-molecule FRET (smFRET) data used to quantify Hfq-mediated annealing between bacterial sRNAs and mRNAs. It includes fluorescence time traces, extracted dwell times, FRET distributions, and analysis scripts supporting measurements of how seed length, Hfq-binding motifs, and motif spacing affect interaction stability and kinetics. The data enable independent reproduction of the analyses and reuse for modeling RNA–RNA interaction dynamics and RNA chaperone function. Funding: This research was funded by the National Science Centre, Poland, grant no 2022/46/E/NZ1/00462 and an EMBO Installation Grant IG 5730-2024.

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Institutions

  • Miedzynarodowy Instytut Biologii Molekularnej i Komorkowej w Warszawie
    Warszawa

Categories

Single Molecule Imaging

Funders

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