Temperature and Humidity Dependent progression and maturation rate of mosquito
Description
The study involved rearing mosquitoes in simulated temperatures and relative humidity. A total of four hundred (400) eggs of An. arabiensis were obtained from JU TIDRC insectary and reared to adults. Mosquitoes were reared across temperatures ranging from the minimum 14.55°C to maximum 34.35°C and relative humidity spanning from 64% to 86%. Each temperature and humidity combination were replicated four times, with one hundred mosquito eggs placed in petri dishes containing wet filter paper. After a 24-hour period, the eggs were carefully washed and transferred into hatching trays. Throughout the larval stage, adequate nourishment was provided, and observations were recorded until pupation occurred. Subsequently, pupae were relocated into cages, and the emergence of adult mosquitoes was closely monitored until the end of their lifespan. Detailed records were maintained, capturing key parameters such as the number of days required for egg hatching, as well as the survival or mortality rates of eggs hatching, larvae, pupae and adult mosquitoes under each temperature and humidity condition.
Files
Steps to reproduce
Rearing of An.arabiensis mosquito carried out both under variable temperatures, relative humidity and standard insectary conditions. Mosquito rearing under simulated condition (variable temperature and humidity) The study involved rearing mosquitoes in simulated temperatures and relative humidity. A total of four hundred (400) eggs of An. arabiensis were obtained from JU TIDRC insectary and reared to adults. Mosquitoes were reared across temperatures ranging from the minimum 14.55°C to maximum 34.35°C and relative humidity spanning from 64% to 86%. Each temperature and humidity combination were replicated four times, with one hundred mosquito eggs placed in petri dishes containing wet filter paper. After a 24-hour period, the eggs were carefully washed and transferred into hatching trays. Throughout the larval stage, adequate nourishment was provided, and observations were recorded until pupation occurred. Subsequently, pupae were relocated into cages, and the emergence of adult mosquitoes was closely monitored until the end of their lifespan. Detailed records were maintained, capturing key parameters such as the number of days required for egg hatching, as well as the survival or mortality rates of eggs hatching, larvae, pupae and adult mosquitoes under each temperature and humidity condition. Mosquitoes were carefully reared under controlled conditions, maintaining a standard temperature and humidity. For adult mosquitoes, the environment was set at a consistent 25±2°C with a relative humidity of 80±10%. Larvae and pupae were nurtured at a temperature of 31±2°C, also with a relative humidity of 80±10%. In this controlled setup, meticulous records were kept of various parameters, including the number of days required for egg hatching, as well as the survival or mortality rates of eggs hatching, larvae, pupae and adult mosquitoes. This standardized environment served as the benchmark, providing a baseline against which the results obtained from simulated environmental conditions could be compared. The rearing of mosquitoes happened under variable temperatures and relative humidity. Totally four hundred (400) eggs of An*. arabiensis* mosquitoes were obtained from JU TIDRC Insectary and reared in temperatures between 14.55-34.35 ℃ and relative humidity (86-64%) at upper and lower temperatures, relative humidity*.* Four replicates of 100 mosquito eggs in petri dishes with wet filter paper were allowed to breed in the provided room at JU TIDRC (Plate 1). Based on temperature and relative humidity, at different time eggs are washed with distilled water. The filter paper was transferred into the hatching tray. Then, eggs hatched to L1 and did not hatch; also, days required hatches to L1 were recorded.
Institutions
- Jimma UniversityOromiya, Jimma