Data supporting a closed-loop computational and experimental framework for prioritizing phage-derived receptor-binding proteins for Yersinia enterocolitica recognition and magnetic enrichment

Description

This dataset supports the Food Control manuscript entitled “A closed-loop computational and experimental framework for prioritizing phage-derived receptor-binding proteins for Yersinia enterocolitica recognition and magnetic enrichment.” It contains the frozen G_pre1 pre-feedback rankings, the separate G_pro1 post-feedback rankings, public scoring policies and audit records, seven C_verified wet-lab evidence records for Q9T0Z9/Gp17, whole-cell ELISA source and plotting data, and magnetic-enrichment source data and calculations. The author-verified original GraphPad Prism one-site binding analysis yielded an apparent Kd of 44.38 ± 2.98 μg/mL (estimate ± SE; R² = 0.9904). An independent unweighted refit of the 36 deposited corrected technical-replicate values reproduced Kd = 44.3785 ± 2.9804 μg/mL and R² = 0.9904206. The separate 12-mean refit is retained only as a secondary numerical check and does not replace the original Prism analysis. Q9T0Z9/Gp17 is the only candidate with direct C_verified wet-lab evidence in this release. The wet-lab evidence concerns bacterial recognition and magnetic enrichment rather than antibacterial activity. Historical ELISA source workbooks and source-code components directly related to a pending patent application are not included. File organization, field definitions, manuscript mapping, analytical scope, limitations, and SHA-256 verification instructions are provided in README.md.

Steps to reproduce

1. Read README.md and review the directory structure and manuscript mapping. 2. Verify the 30 deposited non-manifest files against FILE_MANIFEST_SHA256.csv. 3. Inspect the frozen G_pre1 Top5 and Top50 rankings, scoring rulebook, and validation report. 4. Review the ELISA replicate-level and plotting files and apply the documented background-correction rule. Fit all 36 corrected technical-replicate OD450 values, without weighting, to Y = Bmax × X / (Kd + X). The author-verified original GraphPad Prism output was Kd = 44.38 ± 2.98 μg/mL (estimate ± SE), with R² = 0.9904; the deposited values independently reproduce this result at the reported precision. 5. Treat the independent 12-mean ELISA refit only as a secondary numerical check; it does not replace the original Prism analysis. 6. Inspect the seven Q9T0Z9/Gp17 evidence records in C_verified and the separate G_pro1 post-feedback rankings and audit records. 7. Reproduce the magnetic-enrichment calculation as 85/(12.5 + 85) × 100% = 87.2%. 8. Do not infer unrecorded dilution factors, plating volumes, CFU/mL values, or unavailable source-code operations.

Institutions

• Jilin University

  Jilin, Changchun

Categories

Funders

• Ministry of Science and Technology of the People's Republic of China

  State Council of the People's Republic of China

  Beijing

  Grant ID: 2023YFF0611502
• Jilin Province Science and Technology Department

  Changchun

  Grant ID: 20260203070SF

Licence