Lipidomic raw data
Description
Our aim was to compare the lipidome of SH-SY5Y cells incubated with dACSL4. Our first aim was to determine if AA-phospholipids are regulated in dACSL4-treated cells. There are 3 biological replicates per condition. Cells were incubated with 1 μM dACSL4 or an equivalent volume of DMSO for 12 h, then harvested by trypsinization (500 μL trypsin), diluted with 500 μL PBS, transferred to 1.5 mL tubes, and centrifuged at 800 rpm for 5 min at 4°C. The organic phase was collected and evaporated in a SpeedVac vacuum concentrator. Lipid extracts were dissolved in 100 μL of infusion mixture consisting of 7.5 mM ammonium acetate dissolved in propanol:chloroform:methanol [4:1:2 (vol/vol)]. Samples were analyzed by direct infusion in a QExactive mass spectrometer (Thermo Fisher Scientific) equipped with a TriVersa NanoMate ion source (Advion Biosciences). 2 µL of sample was infused with gas pressure and voltage set to 1.25 psi and 0.95 kV, respectively. All data was acquired in centroid mode. All lipidomics data were analyzed with the lipid identification software, LipidXplorer 29. Tolerance for MS and identification was set to 2 ppm. Data post-processing and normalization to internal standards were done manually in Excel.
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Institutions
- Institute of ChemistryBeijing, Beijing