DATA SET FOR QUANTIFICATION OF TOTAL PHENOLIC COMPOUNDS, FLAVONOIDS, ANTIOXIDANT ACTIVITY, IN VITRO ENZYMATIC INHIBITION AND HEMOLYTIC ACTIVITY OF ANDIROBA (CARAPA GUIANENSIS AUBL) INDUSTRIAL WASTE
Description
This study demonstrated the bioactive potential of the ethanolic extract of the industrial residue of andiroba (Carapa guianensis), focusing on its pharmaceutical applicability in enzyme inhibition. Analyses were performed to quantify total phenols and flavonoids, in addition to in vitro assays to evaluate the antioxidant activity by the DPPH and FRAP methods and the inhibition of the enzymes α-amylase and lipase. The amount of phenols and flavonoids in the sample did not show significant results. However, the extract showed antioxidant activity by the DPPH method, with inhibition of 45.16 ± 7.43% at a concentration of 10 mg/mL and IC50 of 11.57 ± 1.05 mg/mL. The FRAP method showed antioxidant capacity, with inhibition of 238.90 ± 8.78% and IC₅₀ of 0.51 ± 0.07 mg/mL. Furthermore, the extract showed α-amylase inhibitory activity, reaching 65.38% inhibition at 25 mg/mL, with IC₅₀ of 17.52 ± 4.57 mg/mL. The extract also demonstrated inhibition of the lipase enzyme with a percentage of 53.17% at 25 mg/mL and IC₅₀ of 19.44 ± 0.16 mg/mL. These findings suggest that the extract contains compounds capable of modulating the enzymatic activity involved in the digestion of carbohydrates and lipids, which may contribute to postprandial glycemic control. Therefore, the ethanolic extract of andiroba residue shows promise for antidiabetic strategies and highlights the sustainable use of industrial waste with pharmaceutical potential.
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Steps to reproduce
To obtain the ethanolic extract of the industrial residue of andiroba (AIR), the extraction was performed using a Soxhlet extractor, the cycle lasted 8 uninterrupted hours and the solvent evaporation process was carried out in a rotary evaporator. The folin-ciocalteu method was used to quantify the total phenol contents, and the aluminum chloride method was used to quantify flavonoids. The DPPH and FRAP methods were used to analyze the antioxidant potential. The enzyme inhibition assays were performed using α Amylase and lipase. Hemolytic activity was also performed. Data analysis was performed and the data obtained were expressed as mean ± standard deviation of the mean. Statistical analysis was performed using the GraphPad Prism program, version 6.0. The Student's t test was used to analyze the results of the total phenol/flavonoid content and the antioxidant and enzymatic activity. Data with p < 0.05 were considered significant.
Institutions
- Universidade Federal do Oeste do Para