Raw LC–MS/MS proteomic dataset of testicular tissue from control, obese, and diabetic adult male rats

Published: 26 February 2026| Version 1 | DOI: 10.17632/4rfgddv365.1
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Description

Study question: Do obesity and diabetes alter testicular metabolic pathways and reproductive protein abundance in adult male rats? This dataset presents proteomic data derived from a controlled experimental study including adult male Wistar rats distributed into three groups: control, diabetic, and obese (n = 5 per group). Obesity was induced over 38 weeks using a high-calorie diet supplemented with 5% sucrose in drinking water (ad libitum). Diabetes was induced 53 days prior to euthanasia by a single intraperitoneal streptozotocin injection (35 mg/kg). After euthanasia, testicular tissues were carefully dissected, immediately processed for protein extraction, and prepared for shotgun LC–MS/MS proteomic analysis. Data files are structured into separate folders according to experimental group (control, diabetic, and obese), and each folder contains the individual raw mass spectrometry files from the corresponding biological replicates.

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Raw spectra were acquired using MassLynx v4.1 and processed into .mgf files. Protein identification was performed with Mascot Distiller (MDRO 2.4.0.0) against the UniProtKB database for Rattus norvegicus. Relative protein abundance was estimated using the exponentially modified Protein Abundance Index (emPAI). Testicular tissue from adult male Wistar rats (control, diabetic and obese) was analysed by LC–MS/MS (Orbitrap Fusion Lumos). Multivariate analysis included principal component analysis (PCA) and PERMANOVA (999 permutations). Differential abundance was assessed using one-way ANOVA with false discovery rate correction (FDR < 0.05), followed by Fisher’s LSD post hoc testing. Volcano plots were generated using log₂ fold change thresholds (≥1). Functional enrichment analyses were performed using KEGG, Metascape, and ShinyGO.

Institutions

Categories

Proteomics, Metabolic Disorder, Male Reproduction, Translational Research

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