Functional gene profiles (KEGG KO) related to C, N, P, and S cycling in soils under native and Eucalyptus afforestation
Description
This dataset includes KEGG Orthology (KO) functional gene profiles related to carbon (C), nitrogen (N), phosphorus (P), and sulfur (S) cycling in soils from native and Eucalyptus afforestation plots in subtropical China.
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Methods Soil samples were collected from four types of afforestation plantations (Eucalyptus and three native species: Castanopsis hystrix, Albizia falcataria, and Bauhinia variegata) in subtropical China. Each type included four replicate plots (n = 16). Soil microbial DNA was extracted using the FastDNA Spin Kit for Soil (MP Biomedicals, USA) following the manufacturer’s protocol. Metagenomic libraries were prepared and sequenced on the Illumina NovaSeq 6000 platform (paired-end, 150 bp). Raw reads were quality-filtered using Trimmomatic v0.39 and assembled with MEGAHIT v1.2.9. Gene prediction was performed using Prodigal v2.6.3, and functional annotation was conducted against the KEGG database using DIAMOND v2.0.15. KO (KEGG Orthology) profiles were summarized and normalized as relative abundances to represent functional genes related to carbon (C), nitrogen (N), phosphorus (P), and sulfur (S) cycling. The four Excel files in this dataset provide KO abundance data grouped by the four biogeochemical cycles (C, N, P, S).