Using fluorescence anisotropy to monitor chaperone dispersal of RNA-binding protein condensates

Published: 17 May 2022| Version 1 | DOI: 10.17632/4vzph2f83y.1
Contributor:
Haneul Yoo

Description

This README file is for the dataset used in "Protocol for using fluorescence anisotropy to monitor chaperone-dependent dispersal of heat-induced RNA-binding protein condensates" published in STAR Protocols (Yoo and Drummond, 2022). The file contains example data and custom R (version 3.5.2) scripts for analyzing the example data as described in the protocol.

Files

Steps to reproduce

To use this data, unzip the compressed file so that both the raw data and the R code are in the same folder. Go through the chunks in the R code to process, analyze, and plot the data.

Institutions

University of Chicago

Categories

Heat Shock Protein, Chaperone Protein, Biochemistry Methods, Fluorescence Assay, In Vitro Study

Licence