DNA-TFAM smFRET
Published: 15 August 2023| Version 1 | DOI: 10.17632/4whngps32r.1
Contributors:
Hyun Huh, Jiayu Shen, Aparna Ramachandran, Yogeeshwar Ajjugal, , Description
Representative raw microscopy image stacks of donor (Atto565, right panel) and acceptor (Atto67, left panel) fluorescences for LSP, HSP, and NS upon TFAM addition are provided in TIFF format. For each DNA, multiple image stacks (typically 1 ~ 3) were acquired from different sample areas to extract many single-molecule time traces for analysis. The time traces that were used to make Figure 1D-F and 3A-F are provided. Triplicate data sets were further used to obtain the mean and the standard deviation of the kinetic parameters reported in Figure 3G-K and Table S1.
Files
Steps to reproduce
TIFF stacks can be opened by ImageJ. Single-molecule donor-acceptor time series can be opened by ebFRET.
Institutions
Rutgers University Libraries
Categories
Fluorescence Microscopy
Funding
U.S. Department of Energy
DE-SC0019313
National Institutes of Health
GM118086