MELANOCORTIN RECEPTOR ACCESSORY PROTEIN 2-INDUCED ADRENOCORTICOTROPIC HORMONE RESPONSE OF HUMAN MELANOCORTIN 4 RECEPTOR

Published: 21 November 2018| Version 1 | DOI: 10.17632/4wpb5rjn76.1
Contributors:
Lucia Soletto,
Sergio Hernández Balfagó,
Ana Rocha,
Gunnar Kleinau,
Patrick Scheerer,
Jose Miguel Cerda-Reverter

Description

Human (h) and zebrafish (zf) melanocortin receptor 4 (MC4R) activation data. Our data shows how both h and zfMC4R increase the sensitivity to the adrenocorticotropic hormone (ACTH) when interacting with the melanocortin receptor two accessory protein type 2 (MRAP2). Therefore, MC4R becomes an ACTH receptor in the presence of MRAP2 thus opening new gates to the stress effects mediated by ACTH. This MRAP2-induced ACTH sensitivity of MC4R is evolutionary conserved (zebrafish-human) thus suggesting a physiological role for the MC4R/MRAP2 interaction

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Melanocortin receptors were transfected alone or in combination with zfMRAP2 or hMRA2 in HEK-293 cell line stably expressing a reporter gene (Galactosidase) under the control of tandem repetitions of CRE sites (cAMP-responsive elements) and stimulated with ACTH or MSH. Gal activity was expressed as percentage of the basal (no stimulated transfected cells) and data fitted to dose response curves.

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Health Sciences

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