Proton perception and activation of a proton-sensing GPCR

Published: 2 April 2025| Version 1 | DOI: 10.17632/4ycyg3tnpm.1
Contributor:
Yan Zhang

Description

GPR4 is a prototypical proton-sensing GPCR that belongs to Class A GPCRs. We reported the cryo-electron microscopy structures of human GPR4 in its apo inactive, antagonist-bound inactive, Gs-coupled, and Gi-coupled active states. Here, we present SDS-PAGE analysis results for the purified apo inactive GPR4, antagonist-bound inactive GPR4, Gs-coupled active GPR4, and Gi-coupled active GPR4.

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Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis

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