NAT10 drives hepatocellular carcinoma progression through SQLE-mediated cholesterol biosynthesis and is targetable by remodelin
Description
Total RNA was isolated from subcutaneous tumor tissues of mice bearing tumors derived from the MHCC97H human HCC cell line, including samples from the NAT10 knockdown group (sgNAT10) and the negative control group (sgControl). Each group was comprised of four biological replicates, and mRNA sequencing was performed by Novogene (Shanghai).
Files
Steps to reproduce
Following RNA purification and fragmentation, the mRNA templates were converted to complementary DNA (cDNA) via reverse transcription. The resulting cDNA fragments were subsequently ligated to sequencing adapters and amplified using PCR. The final library products were sequenced on the Illumina NovaSeq 6000 platform to generate paired-end reads. Raw sequencing data in FASTQ format were processed using proprietary Perl scripts to yield high-quality clean reads. These processed reads were then aligned to the reference genome utilizing HISAT2 software (v2.1.0) for genomic annotation.
Institutions
- Harbin Medical University Fourth Hospital