Herrera JL-Cell Reports
Published: 22 January 2024| Version 2 | DOI: 10.17632/57mv55r8ys.2
Contributor:
Jose Luis HerreraDescription
RNA-seq raw data from HUVEC cells transducer with Mock or R-Ras38V lentiviruses.
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HUVEC was cultured in the EGM-2 growth medium. 2x10(5)/well seeded on the culture plate at day 0. RNA isolation was performed at day 3. Triplicated FPKM was determined. Sequence reads were subjected to a variety of pre- and post-alignment QC measures before being mapped against the reference genome, hg19, using STAR-2.5.3a. Gene-level quantification was determined using HTSeq 0.6.1 by summation of raw counts of reads aligned to the region associated with each gene. The average read count was 47 million reads. Differential expression analysis was performed using DESeq2_1.6.3. with batch correction.
Institutions
Johns Hopkins University
Categories
Endothelial Cell