Waste-Derived Silver and Lignin Nanoparticles in PVA/Chitosan Hydrogels for Antimicrobial Wound Dressings Dataset
Description
Experimental dataset (UV-Vis, FTIR, XRD, TGA, SEM/EDS, antibacterial and cytotoxicity assays) for PVA/chitosan hydrogels loaded with waste-derived silver and lignin nanoparticles, developed as multifunctional antimicrobial wound dressings.
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Steps to reproduce
AgNPs were green-synthesized from spent black tea leaf extract as reducing/capping agent, and LNPs were obtained from sugarcane bagasse lignin by antisolvent precipitation. Seven PVA/chitosan hydrogel formulations, varying in AgNP, LNP, and gentamicin content, were prepared by physical crosslinking through freeze–thaw cycles. Nanoparticle formation and hydrogel composition were characterized by UV-Vis spectrophotometry, atomic force microscopy (AFM images processed in Gwyddion for particle size, height, and surface roughness analysis), FTIR, XRD, TGA, and SEM/EDS. Swelling kinetics, gel fraction, and PBS mass retention were measured gravimetrically over 7 days. Antibacterial activity against E. coli and S. aureus was assessed by the disk-diffusion method (ampicillin as reference), and cytocompatibility was evaluated by MTT assay on NIH/3T3 fibroblasts.
Institutions
- Universidad Yachay TechImbabura, Urcuquí