Molecular Characterization of Ascaris sp. based on mitochondrial cox1 gene sequence from Pakistani Population

Description

Sequencing Technology :: Sanger dideoxy sequencing source 1..409 /organism="Ascaris sp." /organelle="mitochondrion" /mol_type="genomic DNA" /isolate="Pk_04" /host="Homo sapiens" /db_xref="taxon:27836" /geo_loc_name="Pakistan" /collection_date="2024" gene <1..>409 /gene="COX1"

Steps to reproduce

Primers: Cox1F (5' TTTTTTGGTCATCCTGAGGTTTAT 3') and Cox1R (5' ACATAATGAAAATGACTAACAAC 3') Sequencing Technology :: Sanger dideoxy sequencing Cox1 and regions were assembled using Sequencher 5.4.6 software, and sequences were compared against the GenBank database using NCBI BLAST (http://blast.ncbi.nlm.nih.gov/Blast.cgi). Intraspecific variations were assessed using CLUSTALW (https://www.genome.jp/tools-bin/clustalw).

Institutions

• Quaid-i-Azam University

  Islamabad, Islamabad

Categories

Funders

• Quaid-i-Azam University Islamabad Pakistan

  Grant ID: Internal university research fund

Licence