IAA34-PUCHI

Published: 18 February 2025| Version 1 | DOI: 10.17632/6249ddh7z5.1
Contributor:
Yansong Fu

Description

Western blot of a GST pull down assay with GST-PUCHI and His-MBP-IAA34, using purified GST, GST-PUCHI, and His-MBP-IAA34 expressed in Escherichia coli. His-MBP-IAA34 proteins were incubated with immobilized GST-PUCHI or GST, and the proteins immunoprecipitated with GST beads were detected using anti-His antibody. GST-PUCHI was detected with anti-GST antibody, and His-MBP-IAA34 proteins were detected with anti-His antibody. Three independent repeats show similar results.

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Institutions

Nanjing Agricultural University

Categories

Western Blot

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