Data of characteristics related to morphology, components and wettablity of the epidermis of a butterfly species

Published: 24 February 2025| Version 1 | DOI: 10.17632/6cwwrn6z5v.1
Contributor:
政宇 卢

Description

A comprehensive investigation was conducted to characterize the integumentary features of third-to-fifth instar Papilio polytes larvae, with systematic analysis encompassing three critical parameters: morphological architecture, biochemical composition, and surface wettability. Morphometric evaluation was performed through high-resolution scanning electron microscopy (SEM) to quantify ultrastructural details of the larval epidermis. Compositional profiling was achieved using Fourier-transform infrared (FTIR) spectroscopy in the mid-infrared range (4000-400 cm⁻¹), enabling precise identification of chitinous and proteinaceous components. Hydrophobic properties were quantitatively assessed through contact angle measurements employing a goniometer-based static sessile drop method, with subsequent surface energy calculations derived from Owens-Wendt approximation models.

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This study conducted a tripartite characterization of the epidermal system in Papilio polytes larvae across third to fifth instars, integrating ultrastructural, compositional, and interfacial analyses. Morphological quantification was achieved through high-resolution field-emission scanning electron microscopy (Zeiss EVO-LS10, Gottingen, Germany) following standardized arthropod preparation protocols, including ethanol dehydration gradients naturally air-dried, Biochemical profiling utilized mid-infrared Fourier-transform infrared spectroscopy ( Thermo Fisher Scientific Nicolet iS50, Madison, USA ) with attenuated total reflectance (ATR) mode to identify chitin-protein complexes through spectral deconvolution of amide I/II bands (1700–1500 cm⁻¹). Hydrophobic properties were systematically evaluated via static sessile drop contact angle measurements (KRÜSS DSA100S, Hamburg, Germany) under controlled laboratory conditions (23°C, 50% RH), employing polar (deionized water) and apolar (diiodomethane) probe liquids to calculate surface energy components using the Owens-Wendt thermodynamic model.

Institutions

  • Jiangsu Academy of Agricultural Sciences
  • Shandong Agricultural University

Categories

Insect, Plant Protection, Agricultural Entomology

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