Effects of different concentrations of inorganic phosphate versus parathyroid hormone on osteoblasts
Description
To investigate the effects of inorganic phosphate (P), parathyroid hormone (PTH), and their combined stimulation on proliferation, apoptosis and bone metabolism regulation of osteoblasts, providing a basis for the prevention and treatment of secondary osteoporosis in MHD patients.The MC3T3-E1 osteoblast cell line was divided into blank control group (CON), 2-4mM P single stimulation group, 10-9~-6M PTH single stimulation group, HP-HPTH combined stimulation group and LP-LPTH, LP-HPTH, HP-LPTH treatment group. Each group was continuously stimulated for 24 hours. Proliferation activity, apoptosis rates and the expression of BAP, RUNX2, OPG,and RANKL was detected.
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The MC3T3-E1 osteoblast cell line was divided into blank control group (CON), 2-4mM P single stimulation group, 10-9~-6M PTH single stimulation group, HP-HPTH combined stimulation group and LP-LPTH, LP-HPTH, HP-LPTH treatment group. Each group was continuously stimulated for 24 hours. Proliferation activity, apoptosis rates and the expression of BAP, RUNX2, OPG,and RANKL was detected.
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Tianjin Municipal Science and Technology Commission
TJWJ2021ZD005
Tianjin Municipal Science and Technology Commission
23JCYBJC01330
Tianjin Municipal Science and Technology Commission
23JCQNJC01430
Tianjin Municipal Health Commission
TJSJMYXYC-D2-036
Tianjin hospital
TJYYQ2405
tianjin hospital
TJYYQ2402