Chikungunya virus infection disrupts MHC-I antigen presentation via nonstructural protein 2
Description
Arthritogenic alphaviruses, including chikungunya virus (CHIKV), are re-emerging global public health threats with no approved vaccines or antiviral therapies. Infection with these viruses causes debilitating musculoskeletal disease for months to years that is associated with the persistence of viral RNA and antigen. Prior studies using a mouse model found that CD8+ T cells, which recognize viral peptides in the context of major histocompatibility class I (MHC-I) displayed on the surface of infected cells, have a limited role in the control and clearance of CHIKV infection in joint-associated tissues, suggesting that CHIKV-infected cells evade these critical effectors of the antiviral immune response. Here, we show that MHC-I antigen presentation is inefficient in CHIKV-infected joint tissue fibroblasts, and that a protein encoded by CHIKV and produced in infected cells, nonstructural protein 2 (nsP2), disrupts the surface display of MHC-I molecules and antigen recognition of infected cells by CD8+ T cells. Our findings support a role for CHIKV nsP2 in the evasion of the CD8+ T cell response and establishment of persistent infection.
Files
Institutions
- University of Colorado Denver - Anschutz Medical Campus