Cancer-associated mesothelial cells are regulated by the anti-Müllerian hormone axis

Published: 3 July 2023| Version 1 | DOI: 10.17632/6xwgzcbd28.1
Contributor:
Maeva Chauvin

Description

Original western blot images were obtained from mouse primary mesothelial cells (MPMC) using antibodies against Amhr2 (Santa Cruz, sc-377413), p-Smad1/5 (Cell Signaling, (Ser463/465) (41D10)), and beta-actinin (Cell Signaling, 13E5). Additionally, original western blot images were acquired from the human mesothelial cell line (Met5a) treated with media from cancer cells (BI or Ov90) or transfected with variants of Amhr2 (V1, V3). Staining was performed using antibodies against Amhr2 (Cell Signaling, #4518), p-Smad1/5 (Cell Signaling, (Ser463/465) (41D10)), and beta-actinin (Cell Signaling, 13E5). The western blots included the Precision Plus Protein Dual Color Standards ladder (Bio-Rad #1610374, 10-250 kDa).

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Institutions

Massachusetts General Hospital

Categories

RNA Sequencing, Western Blot

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