n3-Polyunsaturated fatty acids drive differentiation of H3K27M glial progenitors and restrict glioma growth

Published: 11 September 2024| Version 1 | DOI: 10.17632/6zbg352m25.1
Contributor:
Pruthvi Gowda

Description

Lipidomic data from cells and media

Files

Steps to reproduce

Prior to extraction, a 13-class lipid class Lipidizer Internal Standard (AB Sciex, 5040156) was added to each sample. After two extractions, the pooled organic layers were dried down in a Genevac EZ-2 Elite, and lipid samples were resuspended in 1:1 methanol/dichloromethane with 10 mM Ammonium Acetate. Samples were analyzed on the Sciex Lipidyzer Platform for targeted quantitative measurement of 1100 lipid species across 13 classes. Differential Mobility Device on Lipidyzer was tuned with SelexION tuning kit (Sciex 5040141). Data analysis was performed according to the workflow published by Su, B. et al (2021).

Institutions

University of California Los Angeles, Dana Farber Cancer Institute, Harvard Medical School

Categories

Lipidomics, Glioma

Funding

National Institutes of Health

U01CA286537

National Institutes of Health

R01NS121319

Licence