Impact of Insulin-like growth factor-1 (IGF-1) on the aspect ratio and gene expression of rabbit Achilles tenocytes

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We present data on the aspect ratio of rabbit Achilles tenocytes in vitro, based on measuring width and length of the cells, when stimulated with 1, 10 or 100 ng/mL IGF-1 supplementation to culture medium against corresponding cell cultures without IGF-1 supplementation for 3 days. Furthermore, we present for two rabbit donors the impact of 0.1, 1 and 10 ng/mL IGF-1 supplementation to Achilles tenocytes in vitro and of 1 ng/mL IGF-1 that was released from an emulsion electrospun mesh with incorporated IGF-1 to test if the released IGF-1 was still bioactive. Gene expression of target genes collagen I, ki67 and tenomodulin are presented; the data were normalized to gene expression of corresponding cell culture without IGF-1 supplementation.

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