Antioxidant studies of Ruthenium (III) complexes derived from chitosan Schiff base bidentate ligands
Description
3.4.1. DPPH free radical scavenging activity In order to develop metal based anticancer drugs, the transition metal complexes have fulfilled the researcher's expectation to carry out their research in this field. Regards, the ruthenium metal complexes from different kinds of intermediate are playing a versatile role for many biological applications. Also, it is a promising material being used exclusively for some special type of research areas which are primarily in antioxidant study and anticancer, antitumor DNA studies, et c. [41-44]. The series of ruthenium(III) metal complexes derived from chitosan biopolymer based Schiff base bidentate ligands have more potential in its antioxidant activity while compared with other products. Because chitosan is a nontoxic, biodegradable material which has possessed multifaceted character due to the presence of protonated amino groups throughout the polymeric chain [45-47].Thus, efforts were taken to prove antioxidant activity of synthesized products. Accordingly, excellent antioxidant data were obtained and evaluated using suitable drug as standard. The antioxidant potential of ruthenium(III) complexes were measured by DPPH free radical scavenging method at the absorbance of 517 nm on UV-Vis spectrophotometer. From the antioxidant data are given in Table.1, the IC50 values of [Ru(CS-4Hdmb)], [Ru(CS-Hdb)] and [Ru(CS-2Hdmb)] were found to be 16.32 μg/ml, 28.34μg/ml and 18.66μg/ml respectively. All the ruthenium (III) complexes were shown to have an ample scavenging effect on DPPH free radicals. The values of percentage inhibition of ruthenium(III) metal complexes was given in Table 2, which has shown a good free radical scavenging effect at different concentrations. The pictorial representation of scavenging effect of DPPH free radical was given in Fig.11,which was clearly indicated that the all metal complexes has enhanced antioxidant activity and percentage inhibition values are very near to the standard antioxidant drug ascorbic acid. 3.4.2. Lipid peroxidation (LPO) inhibition activity The series of ruthenium(III) complexes were screened for LPO free radical scavenging activity and results were given in Table 3. The data revealed that all bioactive products of bidentate ligands have shown high potential in their scavenging activity on LPO free radicals. The percentage inhibition of [Ru(CS-4Hdmb)] and [Ru(CS-2Hdmb)] complexes show an excellent effect on LPO free radical scavenging activity. But, the [Ru(CS-Hdb)] complex is given moderate effect when compared with other complexes. The LPO inhibitory activities of metal complexes were represented as graphical views in Fig. 12. Trolox was used as a standard.