Whole genome sequencing of 4 carbapenem resistant Acinetobacter baumannii from our tertiary care hospital
Description
This is the fasta file of the whole genome sequencing done on carbapenem resistant Acinetobacter baumannii isolated from the blood of 4 critically ill Patients admitted in ICU of our hospital. Carbapenem resistant Acinetobacter baumannii is one of the most lethal microorganisms responsible for very high mortality and morbidity in Intensive Care Units worldwide. Antimicrobial resistance and virulence are attributable to various genes making the therapeutic options very limited. We aimed to find out the antimicrobial resistance and virulence genes present on carbapenem resistant Acinetobacter baumannii by whole genome sequencing. It was a retrospective observational study done at a tertiary care hospital of Western India from 1st January 2023 to 30th June 2023 on 4 isolates of clinically significant carbapenem resistant Acinetobacter baumannii from adults admitted in ICU with blood stream infection. Whole genome sequencing of these isolates was done to find out the genes responsible for antimicrobial resistance and virulence. Bla oxa 133 was found for carbapenemase production, aadB and aac for aminoglycoside resistance, gyrA, gyrB, abaQ for flouroquinone resistance and emr genes for colistin resistance. Many genes like mdt and emr which were not previously reported in Acinetobacter baumannii were found in all isolates. The patients who were infected with Acinetobacter baumanni having mdt and emr genes had a rapid course of illness. This study found presence of many novel genes for antimicrobial resistance in carbapenem resistant Acinetobacter baumannii isolates of our setup. mdt and emr genes were found in isolates who had increased virulence and lethality.
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Institutions
- All India Institute of Medical Sciences Jodphur