Decitabine-containing regimens could alleviate acute graft-versus-host disease via regulating intestinal epithelial-microbiota-innate lymphoid cells barrier

Description

A Bu/Cy-induced aGvHD mouse model was established in four groups (Non-DAC, DAC, BMT, Control). Post-conditioning, colon RNA/protein were analyzed by RT-qPCR (cytokines, tight junctions, mucins, ILC TFs) and CBA (cytokines); colonic ILC subsets were assessed by flow cytometry. On day +7, clinical scores, pathology, and CD3⁺ T-cell infiltration were evaluated, with repeated cytokine and ILC analyses. Additional aGvHD models (co-housed, pseudo-germ-free, differential colonization) were used for comparison; fecal metabolites were profiled to explore microbiota mechanisms via gut epithelial–microbiota–ILC crosstalk.

Institutions

• Peking University First Hospital

  Beijing, Beijing

Categories

Licence